Restriction Enzyme Digestion

Restriction enzyme - A restriction enzyme (or restriction endonuclease) is an enzyme that cuts double-stranded DNA. The enzyme makes two incisions, one through each of the phosphate backbones of the double helix without damaging the bases.

Restriction sites - Restriction sites, or restriction recognition sites, are particular sequences of nucleotides that are recognized by restriction enzymes as sites to cut the DNA molecule. The sites are generally palindromic, and a particular enzyme may cut between two nucleotides within its recognition site, or somewhere nearby.

Restriction digest - A restriction digest is a molecular biology procedure used to prepare DNA for analysis or other processing. Also known as DNA fragmentation, it uses a restriction enzyme to selectively cleave strands of DNA into shorter segments, which are more suitable for analytical techniques such as chromatography.

Restriction fragment - A DNA fragment resulting from cleavage of a DNA strand by a restriction enzyme. Restriction fragments can be analyzed using techniques such as gel electrophoresis.

restrictionenzymedigestion

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